Effectiveness of Solidifying Agent and Haustorium Removal on Kopyor Coconut Zygotic Embryo Culture

2011 Perhorti Seminar

Siti Halimah Larekeng - PhD/S3 student at the PMB Lab presented oral presentation on zygotic embryo rescue of Kopyor coconut at 2011 Perhorti Seminar, 23-24 November 2011, Balitsa-Lembang.

(Kefektifan Bahan Pemadat Dan Pemotongan Haustorium Pada Kultur Embrio Zigotik Kelapa Kopyor)

Siti Halimah Larekeng, Nurhayati AA. Mattjik, Agus Purwito, dan Sudarsono

Lab. Biologi Molekuler Tanaman (PMB Lab), Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB), Jl. Meranti – Kampus IPB Darmaga, Bogor 16680. Email: s_sudarsono@ymail.com

 ABSTRACT

Constrains in obataining homozygote Kopyor coconut seedlings are: the homozygote kopyor coconut fruits are not able to germinate and the alternative methods for producing Kopyor coconut seedlings through zygotic embryo culture are relatively too long. The objectives of this experiments were to improve Kopyor coconut zygotic embryo culture methods capable of producing seedlings in a shorter period than the available methods. In this experiments, effects of solidifying agent and removal of haustorium in Kopyor coconut zygotic embryo culture were evaluated. Kopyor coconut zygotic embryos, with or without haustorium, were cultured on Y3 medium supplemented with 5 ppm BAP. Agar at 5 or 7 g/l were added in the Y3 medium. The zygotic embryos of Kopyor coconut were cultured on the evaluated media and their growth and development were observed. Results of the experiments indicated that removal of haustorium from Kopyor coconut zygotic embryos resulted in faster shoot initiation and growth from the explant than that of the complete embryos. Similarly, first root initiation were also better for zygotic embryos without haustorium. Addition of agar at 5 mg/l is more effective than that of 7 g/l to induce further growth from Kopyor coconut zygotic embryos.

Keywords: Kopyor coconut propagation, Heterozygote Kopyor coconut, Zygotic embryo rescue.

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This paper was presented at: 2011 Indonesian Horticulture Society (Perhimpunan Hortikultura Indonesia [PERHORTI] Seminar, Lembang. Bandung – 23-24 November 2011.

About PMB Lab: Prof. Sudarsono

This blog is dedicated as a communication media among alumni associated with PMB Lab, Dept. of Agronomy and Horticulture, Fac. of Agriculture, IPB, Bogor – Indonesia. It contains various information related to alumni activities, PMB Lab’s on going activities and other related matters.
This entry was posted in Coconut (Cocos nucifera), Kopyor Coconut, News from PMB Lab, Oral Presentation, PhD (S3) Student, Scientific Seminar and tagged , . Bookmark the permalink.

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