Two new DNA sequences isolated from tobacco (Nicotiana tabaccum) have just been published in the NCBI DNA Database. The two DNA sequences were submitted by Sesanti Basuki, a PhD graduate student working at the PMB Lab, Department of Agronomy and Horticulture, Faculty of Agriculture, IPB. The two DNA sequence records were officially released for publication in the NCBI database in May 9, 2012.
One of the DNA sequence was part of putrescine N-methyl transferase (PMT) gene isolated from local Temanggung tobacco cultivar cv. Sindoro 1. The partial PMT gene database accession number was JQ438825. While the other was part of quinolinate phosphoribosyltransferase (QPT) gene isolated from the same local tobacco cultivar. The partial QPT gene database accession number was JQ518218.
Isolation of genes associated with nicotine biosynthesis and their use for modifying nicotine content in local tobacco varieties are part of the topics of Sesanti Basuki’s PhD dissertation research. After isolating the genes, she will construct expression vectors (transgenes) carrying isolated gene to express either antisense RNA or RNAi and generate transgenic tobacco carrying the transgenes. Once regenerated, the transgenic plants will be used to study possibilities of having reduced nicotine content but without any changes in other attribute of tobacco leaf qualities.