Keragaman dan Hubungan Genetik antara Kelapa Tetua Genjah Kuning Nias (GKN) dan Dalam Tenga (DTA) serta Hibrida KHINA-1 Berdasarkan Marka Mikrosatelit

Pesik, A.; Effendi, D.; Novarianto. H., Dinarty, D.;  Maskromo, I; Tenda, Elsje T.; Sudarsono. 2014. Keragaman dan Hubungan Genetik antara Kelapa Tetua Genjah Kuning Nias (GKN) dan Dalam Tenga (DTA) serta Hibrida KHINA-1 Berdasarkan Marka Mikrosatelit [Genetic Diversity and Association among Nias Yellow Dwarf (NYD), Tenga Tall (TAT) and KHINA-1 Hybrid Coconuts Based on Microsatellite Markers]. Bulletin Palma 15(2):129-140

ABSTRACT

Information on genetic diversity and association among parent and progenies plays an important role in plant breeding. Genetic identity among progenies derived from controlled population need to be determined to make sure that they are from hybridization of parents and they are not because of pollen contamination (author: from open pollination). Microsatellite markers can be used to evaluate genetic diversity and relationship among parents and their hybrid populations. The objectives of this study are to evaluate the genetic diversity of NYD, TAT and KHINA-1 hybrid populations; and to analyze the genetic relationship among NYD and TAT as parents, with the KHINA-1 hybrid populations using 19 microsatellite markers. The results showed that the polymorphic information content (PIC) of 19 microsatellite marker loci was ranged from 0.18-0.72 and the average allele per locus was 3.68.

Based on phylogenetic analysis using Neighbor-Joining for tree construction conducted by using DARwin5 Software, individuals of the NYD population belong to a single group; the TAT population are heterogeneous population with the majority of the individuals are belonged into a single group and the rest are belonged to at least two separate groups; and those of KHINA-1 hybrid population are divided into at least  five groups. Estimation of the population structure using STRUCTURE software shows that the presence of high segregation and or recombination event among the KHINA-1 progeny, indicating there has been a mixture of genetic materials from NYD and TAT parents  in the KHINA-1 hybrids. More accurate selection using molecular marker should aid the development of more productive coconut hybrids.

Keywords: Genetic diversity, hybrid coconut, microsatellite marker

ABSTRAK

Informasi keragaman dan hubungan genetik antar tetuua dan progeni penting dalam strateji pemuliaan tanaman kelapa. Identitas genetik antar progeni dari persilangan terkontrol perlu diidentifikasi untuk memastikan bahwa progeni berasal dari tetuanya, dan bukan akibat kontaminasi serbuk sari (Author: hasil pogglinasi terbuka). Penggunaan marka mikrosatelit mampu mendeteksi keragaman genetik aksesi dan menduga kebenaran tetuanya. Tujuan penelitian ini adalah untuk mengevaluasi keragaman genetik populasi tetua GKN dan DTA serta hibrida KHINA-1, dan menganalisis hubungan genetik antara tetua GKN dan DTA dengan hibrida KHINA-1 menggunakan 19 marka mikrosatelit. Hasil penelitian menunjukkan nilai polimorfisme 19 marka mikrosatelit berkisar antara 0.18-0.72, dengan rataan jumlah alel 3.68 per lokus. Berdasarkan hasil analisis filogenetik dengan metode Neighbor-Joining menggunakan program DARwin5, dapat diketahui bahwa populasi GKN tergolong dalam satu group; populasi DTA merupakan populasi yang heterogeneous dengan sebagian besar individu tergabung ke dalam satu kelompok dan sisanya ke dalam minimal dua kelompok yang berbeda; dan populasi hibrida KHINA-1 terbagi ke dalam minimal lima kelompok yang berbeda. Estimasi struktur populasi dengan perangkat lunak STRUCTURE menunjukkan adanya tingkat segregasi dan atau rekombinasi yang tinggi pada populasi KHINA-1, yang mengindikasikan telah terjadi percampuran materi genetik antara tetua GKN dan DTA pada populasi hibrida KHINA-1. Seleksi yang lebih akurat menggunakan marka molekuler dapat membantu perakitan kelapa hibrida yang lebih produktif.

 

Kata kunci: Keragaman genetik, kelapa hibrida, marka mikrosatelit.


Here is how to cite this publication:

Anneke, P.; Effendi, D.; Novarianto. H., Dinarty, D.;  Maskromo, I; Elsje T. Tenda; Sudarsono. 2014. Keragaman dan Hubungan Genetik antara Kelapa Tetua Genjah Kuning Nias (GKN) dan Dalam Tenga (DTA) serta Hibrida KHINA-1 Berdasarkan Marka Mikrosatelit. Bulletin Palma 15(2):129-140.

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Link untuk versi pdf hasil scan copy dari publikasi [The link to Pdf scan copy of the publication] ada di [is] Anneke Pesik Bul Palma 2016

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About PMB Lab: Prof. Sudarsono

This blog is dedicated as a communication media among alumni associated with PMB Lab, Dept. of Agronomy and Horticulture, Fac. of Agriculture, IPB, Bogor – Indonesia. It contains various information related to alumni activities, PMB Lab’s on going activities and other related matters.
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