Category Archives: Q & A

Q&A in AGH635 Course: In RAPD Marker Analysis – What is Called the Locus and What is the Allele?

The term locus (pl.: loci) is a common terminology associated with a location in the genome of an organism. In the Mendelian genetics, the locus controlling flower color clearly describes a location somewhere in the genome associated with gene(s) controlling … Continue reading

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Q&A in AGH635 Course: What is RAPD Marker and How To Generate the Marker?

One of the early polymerase chain reaction (PCR) based DNA markers develops was RAPD (Randomly Amplified Polymorphic DNA) marker. In a Q & A session of the AGH635 lecture, one of the student asked the following question: “What is RAPD … Continue reading

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Q&A in AGH635 Course: Mendelian Segregation to Estimate Number of Locus and Co-segregation to Evaluate Linkage Between Loci

Once my student taking AGH635. Molecular and Cellular Analysis in Plant Breeding Course raised the following case: “I have collected 200 accessions of plants randomly collected from 20 different provinces in Indonesia. The accessions were brought to and evaluated in … Continue reading

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Q&A in AGH635 Course: What Conclusion Can We Draw from Molecular Markers Analysis of a Group of Plant Accessions?

One of my student taking AGH635. Molecular and Cellular Analysis in Plant Breeding Course, raised the following case: “I have analyzed 200 plants for different morphological and yield associated characters. The same plants were also subjected to analysis using 100 … Continue reading

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Q&A in AGH635 Course: Associating Molecular Markers with Phenotypic Characters

A student who took AGH635. Molecular and Cellular Analysis in Plant Breeding Course, once raised the following case: I have analyzed 100 plants for their resistance against a particular plant disease. The same plants were also subjected to molecular analysis … Continue reading

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Q&A in AGH635 Course: Either Common By Descend Or Not!

A student of mine taking AGH635. Molecular and Cellular Analysis in Plant Breeding Course, once asked me the following question: “Is there any specific requirement we should follow about the plant population used in molecular marker analysis and evaluation?” Instead … Continue reading

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The 15 Pages Rules – The Easy Way To Write Scientific Publication in Indonesian Journal

Writing scientific publication to some of us may not be an easy task. Although this notion may be true, writing scientific manuscript should not be a stressful chorus. We should be making it a fun and joyful activities. To you … Continue reading

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Q&A in AGH634 Course: Aneka Pertanyaan tentang Real Time PCR # 2

Salah satu mahasiswa pascasarjana yang pernah mengambil kuliah AGH635. Teknik Molekuler Seluler dalam Pemuliaan Tanaman bertanya pada saya tentang analisis real-time PCR untuk mempelajari ekspresi gen MADS Box dan hubungannya dengan abnormalitas pembentukan buah pada kelapa sawit. Masalah yang dipertanyakan … Continue reading

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Q&A in AGH634 Course: Aneka Pertanyaan tentang Real Time PCR # 3

Salah satu mahasiswa pascasarjana yang pernah mengambil kuliah AGH635. Tenik Molekuler Seluler dalam Pemuliaan Tanaman bertanya pada saya tentang hasil analisis UPLC (Ultra Performance Liquid Chromatography). Masalah yang dipertanyakan adalah sebagai berikut: Saya memakai UPLC untuk mempelajari ekspresi gen MADS-box … Continue reading

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Let’s Talk Mutation #1: Suatu Pengantar – Apa Sih Sebetulnya Mutasi Itu?

Ketika kita membaca atau mendengar istilah “mutasi” pada tanaman maka dalam fikiran kita langsung berasosiasi dengan proses yang menyebabkan terjadinya perubahan fenotipik pada tanaman, sehingga menampilkan fenotipe ‘abnormal.’ Fenotipe ‘abnormal’ (dalam hal ini, fenotipe yang berbeda dengan yang biasa kita … Continue reading

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